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Microscopy & Imaging · 2025

Teach your microscope how to print: low-cost and rapid-iteration microfabrication for biology

Our protocol drastically reduces material costs compared to conventional methods and enables design-to-device turnaround within a day. By leveraging open-source microscope control software and existing lab equipment, our workflow lowers the entry barrier to microfabrication, enabling labs to prototype custom solutions for diverse experimental needs while maintaining compatibility with soft lithography and downstream biological assays.

Performance the paper reports

0 of 5
  1. Motion—Motion accuracy: not reported
  2. Volume—Volumetric accuracy: not reported
  3. Viability—Cell viability: not reported
  4. Unattended—Unattended operation: not reported
  5. Standard—Standard compliance: not reported

The full text was read and states no figure for any of the five.

How hard this is to adopt

4.0 of 5, higher is easier

Each score is shown against the range the other 59 builds actually occupy, because no criterion uses the full 1–5 scale. These describe how reproducible the build is, not how good the science is.

  • Resolution

    5/1–5

    Smallest feature/positioning evidence about 1 µm.

  • Throughput

    4/2–5

    Evidence of parallel, plate-scale, multi-head, or unattended operation.

  • Parts sourcing

    4/2–4

    Mostly off-the-shelf or kit-based components.

  • Skill needed

    4/2–4

    Build/operation described as low-skill or basic assembly.

  • Facilities needed

    4/2–4

    Uses common benchtop/desktop equipment or generic consumables.

  • Documentation

    3/2–5

    Some open documentation or files are available.

  • Validation effort

    4/2–4

    Validation includes standards, benchmarking, replicates, or multi-condition tests.

  • Cycle time

    4/1–4

    Speed evidence suggests rapid, real-time, or automated operation.

2 further criteria are recorded but not shown
  • Application Level — scored 4 (75% of records share one value), so it cannot separate one build from another.
  • Build Time — scored 5 (scored for only 6 of 60 records), so it cannot separate one build from another.

Both remain in the published dataset.

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Record metadata
Publication data

Matched by doi · retrieved 2026-07-21

Abstract

. Our protocol drastically reduces material costs compared to conventional methods and enables design-to-device turnaround within a day. By leveraging open-source microscope control software and existing lab equipment, our workflow lowers the entry barrier to microfabrication, enabling labs to prototype custom solutions for diverse experimental needs while maintaining compatibility with soft lithography and downstream biological assays.

Bibliographic details

Published
2025-01-01
Journal/source
Lab on a Chip
Publisher
Royal Society of Chemistry (RSC)
DOI
10.1039/d5lc00181a
Type
journal-article
Language
en
Volume / issue
25 / 16
Pages
4091-4105
ISSN
1473-0197, 1473-0189

Access and metrics

Open access
Yes
OA status
hybrid
License
cc-by
Version
publishedVersion
Cited by
4
References
63
Retracted
No

Authors and affiliations

  1. Lucien HinderlingCorresponding authorUniversity of Bern
  2. Remo HadornUniversity of Bern
  3. Moritz KwasnyUniversity of Bern
  4. Joël FreiUniversity of Bern
  5. Benjamin GrädelUniversity of Bern
  6. Sacha PsalmonUniversity of Bern
  7. Yannick BlumUniversity of Bern
  8. Rémi BerthozInstitut de génétique et de biologie moléculaire et cellulaire
  9. Alex E. LandoltUniversity of Bern
  10. Benjamin D. TowbinUniversity of Bern
  11. Daniel RivelineCentre National de la Recherche Scientifique · Institut de génétique et de biologie moléculaire et cellulaire · Université de Strasbourg
  12. Olivier PertzCorresponding authorUniversity of Bern

Topics and keywords

Microfluidic and Bio-sensing TechnologiesNanofabrication and Lithography Techniques3D Printing in Biomedical ResearchMicrofabricationPhotolithographyMicroscopeNanotechnologyRepurposingFluorescence microscopeMicrofluidicsMaterials scienceComputer scienceEngineeringOpticsFluorescence

Funding

  • University of Bern · Open Round 2022-4
  • Uniscientia Foundation · 187-2021
  • Novartis Stiftung für Medizinisch-Biologische Forschung · #20C219
  • Experiment · 10.18258/50706
  • Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung · 310030_185376
  • Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung · 310030_207475
  • Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung · CRSII5_183550
  • Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung · PCEFP3_181204
Perspective and practical signals

Why it matters

No narrative note curated yet.

Limitations

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