An Open-Source 3D Bioprinter Using Direct Light Processing for Tissue Engineering Applications

Abstract

The demand for organ transplantation continues to rise worldwide, intensifying the gap between supply and demand and driving research in tissue engineering (TE). Bioprinting, particularly light-based vat photopolymerization (VP) methods such as digital light processing (DLP), has emerged as a promising strategy to fabricate complex, cell-compatible tissue constructs with high precision. In this study, we developed an open-source, bottom-up DLP bioprinter designed to provide a cost-effective and modular alternative to commercial systems. The device was built from commercially available components and custom-fabricated parts, with tolerance allocation and deviation analyses applied to ensure structural reliability. Mechanical and optical subsystems were modeled and validated, and the control architecture was implemented on the Arduino platform with a custom Python-based graphical interface. The system achieved a theoretical Z-axis resolution of 1 μm and a vertical travel range of 50 mm, with accuracy and repeatability comparable to research-grade bioprinters. Initial printing trials using polyethylene glycol diacrylate (PEGDA) hydrogels demonstrated high-fidelity microfluidic constructs with adequate dimensional precision. Collectively, these results validate the functionality of the proposed system and highlight its potential as a flexible, precise, and cost-effective platform that is also easy to customize to advance the democratization of biofabrication in TE.