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Bioprinting · 2024

Development of a microfluidic-assisted open-source 3D bioprinting system (MOS3S) for the engineering of hierarchical tissues

crosslinking and scaffolds fabrication. The coupling of 3D printed syringe pumps with the motion control system is used for driving the tunable extrusion of inks for the fabrication of centimeter scale hierarchical lattice constructs for tissue engineering purposes.

Performance the paper reports

0 of 5
  1. Motion—Motion accuracy: not reported
  2. Volume—Volumetric accuracy: not reported
  3. Viability—Cell viability: not reported
  4. Unattended—Unattended operation: not reported
  5. Standard—Standard compliance: not reported

The full text was read and states no figure for any of the five.

How hard this is to adopt

3.5 of 5, higher is easier

Each score is shown against the range the other 59 builds actually occupy, because no criterion uses the full 1–5 scale. These describe how reproducible the build is, not how good the science is.

  • Resolution

    3/1–5

    Dispensed 50-280 uL (n=3) with Bland-Altman error growing as volume falls; printing flow rates 10-30 uL/min producing 250 µm strands; no pulsation data.

  • Throughput

    4/2–5

    Three 3D-printed syringe pumps on separate stepper drivers addressed independently by G-code via DISTINCT_E_FACTORS, with automated ink switching and unattended 20-layer gradient prints.

  • Parts sourcing

    3/2–4

    Mix of common parts and custom/printed components.

  • Skill needed

    4/2–4

    Build/operation described as low-skill or basic assembly.

  • Facilities needed

    4/2–4

    Uses common benchtop/desktop equipment or generic consumables.

  • Documentation

    4/2–5

    Open resources include several build or operation artifacts.

  • Validation effort

    3/2–4

    Multiple volumes, six hydrogel formulations, collapse test and strand/pore measurement against design, but no experimental benchmark against a commercial bioprinter or pump.

  • Cycle time

    3/1–4

    No timing comparison reported; ink transition took 8 min at 20 uL/min and 16 min at 10 uL/min with no conventional reference.

2 further criteria are recorded but not shown
  • Application Level — scored 5 (75% of records share one value), so it cannot separate one build from another.
  • Build Time (scored for only 6 of 60 records), so it cannot separate one build from another.

Both remain in the published dataset.

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Publication data

Matched by title · retrieved 2026-07-21

Abstract

crosslinking and scaffolds fabrication. The coupling of 3D printed syringe pumps with the motion control system is used for driving the tunable extrusion of inks for the fabrication of centimeter scale hierarchical lattice constructs for tissue engineering purposes. MOS3S performance have been validated to fabricate high-resolution structures with coaxial microfluidic technology, opening to new frontiers for seminal studies in pre-clinical disease modelling and tissue regeneration.

Bibliographic details

Published
2024-04-02
Journal/source
HardwareX
Publisher
Elsevier BV
DOI
10.1016/j.ohx.2024.e00527
Type
journal-article
Language
en
Volume / issue
18
Pages
e00527
ISSN
2468-0672

Access and metrics

Open access
Yes
OA status
gold
License
cc-by
Version
publishedVersion
Cited by
15
References
30
Retracted
No

Authors and affiliations

  1. Sajad MohammadiItalian Institute of Technology · Center for Nano Science and Technology · Sapienza University of Rome
  2. Salvatore D’AlessandroItalian Institute of Technology · Center for Nano Science and Technology · Sapienza University of Rome
  3. Fabiano BiniSapienza University of Rome
  4. Franco MarinozziSapienza University of Rome
  5. Gianluca CidonioItalian Institute of Technology · Center for Nano Science and Technology

Topics and keywords

3D Printing in Biomedical ResearchAdditive Manufacturing and 3D Printing TechnologiesInnovative Microfluidic and Catalytic Techniques InnovationMicrofluidicsNanotechnology3D bioprintingTissue engineering3D printingBiofabricationScaffoldFabricationComputer scienceMaterials scienceBiomedical engineeringEngineering
Perspective and practical signals

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